ULTRASOUND can increase the surface area of the treated biomass and provide sufficient delignification to facilitate the hydrolysis of the carbohydrate components in the subsequent process steps.

Hemicellulose hydrolysis and further matrix degradation can be provided by the action of subcritical and/or supercritical CO2 (SC-CO2).

Enzymatic hydrolysis needs will be tailored to the pre-treatment methods used

Each constituent in the biomass (plant) can and will be functionalized in order to produce non-food and food fractions

The ambition of US4GREENCHEM is to combine only green technologies for the conversion of lignocellulosic biomass into a sustainable biorefinery for chemicals and fuels. The full integration and optimization of multi-frequency ultrasound treatment with supercritical CO2 and enzymatic hydrolysis has not been attempted before.